Do not use on cats. Do not use on puppies under 12 weeks of age. Available in Small Dog up to 15 and Big Dog up to 28 sizes. Active Ingredients Propoxur 10%, Other ingredients 90%. Bio Spot Flea and Tick Collar for Puppies kills fleas and ticks including those carrying Lyme disease. Provides long lasting protection that lasts for five months and starts to work as soon as it is placed around your puppy's neck. Complete protection against fleas will be achieved in a few days. Continues to provide continuous action, even when wet. Replace collar every five months for continuous protection against fleas and ticks. For use on puppies 12 weeks of age and older. Directions Buckle collar around dogspuppy's neck. Fit collar snug enough to prevent placement of the animals leg or lower jaw through the collar, yet loose enough to be rotated about the neck. Leave 2 3 extra inches on the collar for adjustment, cut off excess. Made in the USA.